In Vitro Capacitation of Canine Spermatozoa.
نویسندگان
چکیده
منابع مشابه
Evaluation of in vitro capacitation of stallion spermatozoa.
The primary aim of this study was to establish a flow cytometric technique for determining the capacitation status of stallion spermatozoa. To this end, a flow cytometric technique that demonstrates changes in plasma membrane fluidity; namely, merocyanine 540 staining, was compared with the more conventional Ca(2+)-dependent fluorescence microscopic technique, chlortetracycline (CTC) staining, ...
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The aim of this work was to study the influence of vitamin E (2mM) on capacitation and acrosome reaction of crossbred cattle bull spermatozoa. Fresh semen suspended in TALP medium (pH 7.4) was divided into four equal fractions. All fractions; control (containing TALP + spermatozoa) and the experimental [treated / untreated with ferrous ascorbate (FeAA – 150 μM FeSO4 : 750 μM ascorbic acid); sup...
متن کاملCentrifugation stress reduces the responsiveness of spermatozoa to a capacitation stimulus in in vitro-aged semen.
Density gradient centrifugation of semen is commonly used in many assisted reproduction techniques. Although gradients have the potential to isolate and enrich motile and viable spermatozoa, the centrifugation force presents a stress factor to cell organelles and membranes. The objective of the study was to evaluate the impact of density gradient centrifugation stress on sperm capacitation dyna...
متن کاملCat (Felis catus L.) spermatozoa require capacitation.
In the oestrous female cat, fertilization can take place when spermatozoa are introduced into the vagina up to 49 hr after an ovulation-inducing injection of human chorionic gonadotrophin. The spermatozoa require residence in the female reproductive tract before they are capable of fertilizing the eggs in vitro. No eggs were fertilized by freshly ejaculated spermatozoa. One of four oestrous fem...
متن کاملEvaluation of lipid changes in buffalo (Bubalus bubalis) spermatozoa during in-vitro capacitation and acrosome reaction
In this study, buffalo spermatozoa were in-vitro capacitated and acrosome reacted using modified Tyrode’s bicarbonatebuffered medium (sp-TALP media). Lipids were extracted from freshly ejaculated, in-vitro capacitated and acrosome reacted buffalo spermatozoa. The total lipid content of freshly ejaculated spermatozoa (171.5±2.90 μg/108 cells), capacitated spermatozoa (128.2±3.29 μg/108 cells) an...
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ژورنال
عنوان ژورنال: Journal of Reproduction and Development
سال: 1992
ISSN: 0916-8818,1348-4400
DOI: 10.1262/jrd.38.67